- Abbreviation: PREMISE
- Project code: PN-III-P2-2.1-PED-2019-4018
- Contract number: 524PED/2020
- Start: 23 October 2020
End: 21 October 2022
- Duration: 24 months
- Budget: 600.000,00 RON
- Coordinating institution: UPB-CNMN – University POLITEHNICA of Bucharest –
National Center for Micro and Nanomaterials
Coordinator – University POLITEHNICA of Bucharest
Project Manager: Assoc.Prof.PhD.Eng. Denisa FICAI
Scientific Researcher I: Prof.PhD.Eng. Ovidiu Cristian OPREA
Scientific Researcher III: SRIII.PhD.Eng. Bogdan Ștefan VASILE
PhD student: PhD.Stud.Eng. Cornelia Ioana ILIE
Scientific Researcher III: Lect.PhD.Eng. Ionela Andreea NEACŞU
PhD student: PhD.Stud.Eng. Roxana Doina TRUȘCĂ
PhD student: PhD.Stud.Eng. Georgiana DOLETE
PhD student: PhD.Stud.Eng. Ludmila MOTELICĂ
Scientific Researcher III: Lect.PhD.Eng. Adrian Vasile SURDU
Scientific Researcher: PhD.Eng. Angela SPOIALĂ
PhD student: PhD.Stud.Eng. Alexa Maria CROITORU
Partner I (P1): National Institute for Research & Development in Chemistry and Petrochemistry – ICECHIM
Partner responsible: PhD.Eng. Irina FIERASCU (DUMITRU)
Scientific Researcher I: Phd. Radu Claudiu FIERASCU
Scientific Researcher I: Phd. Valentin RADIȚOIU
Scientific Researcher I: Phd. Cristian Andi NICOLAE
Scientific Researcher II: Phd. Raluca SOMOGHI
Scientific Researcher III: Phd. Elena CHICAN
Scientific research assistant: Phd. Roxana Ioana BRAZDIS
Scientific research assistant: Phd. Ana Maria BAROI
Scientific research assistant: Phd. Fristos TOMA
Partner II (P2): Stefan S. Nicolau Institute of Virology (IVN)
Partner responsible: S.R.I.PhD.Eng. Coralia BLEOTU
Scientific Researcher II: Phd. Lilia MATEI
Scientific Researcher II: Phd. Laura NECULA
Scientific Researcher III: Phd. Denisa DRAGU
Scientific Researcher III: Phd. Ana NEAGU
PhD student: Phd.Stud. Ioana PITICA
Scientific Researcher III: Phd. Otilia BANU
Scientific Researcher III: Phd. Grațiela GRĂDIȘTEANU
The aim of this project proposal is to develop new micro and mesoporous (MMM) materials using recent synthesis methods and to further functionalize these materials in order to improve their efficiency, so that they can be used as controlled release systems, in particular for diseases associated with the microbiota.
These controlled release systems are developed for oral administration and are expected to be protected in the stomach and maintained until the desired target site is reached. Although these controlled release platforms are highly studied in the literature for several types of applications such as controlled release systems (which host antitumor, anti-infective or analgesic agents), the field of microbiota is studied very little.
Nowadays, especially in children, awareness of the importance of the microbiota is well known; therefore, after treatment with antibiotics, it is often necessary to prescribe probiotics. Within the limits of the project, the natural substances used as biologically active agents will be loaded into the porous platforms, to ensure a positive feedback to the microbiota due to its antioxidant, antimicrobial, anti-inflammatory and anticancer activities. In recent years, much emphasis has been placed on the development of micro- and mesoporous materials loaded with biologically active agents, but few scientific papers present the impact of functionalized MMMs on the microbiota.
In this project, the main goal is to develop innovative MMMs with an improved pore system, capable of hosting and releasing biologically active agents (vitamins and polyphenols will be used) for the treatment of microbiota-related diseases.
Because most polyphenols have a low solubility, two innovative controlled release systems will be developed in order to ensure intelligent release (to a target site).
Therefore, in the current project, 3 general objectives (GO), achieved through several specific objectives (SO), have been taken into account, as follows:
GO 1. Exploiting the results of research obtained by applicants for the establishment and development of new innovative delivery systems for biologically active species (medicines). This goal is achievable by pooling preliminary research results on micro and mesoporous materials used as drug delivery systems, along with modern functionalization techniques and biologically active natural agents, resulting in new treatment tools for gastrointestinal microbiota diseases.
GO 2. Laboratory validation of a technology aimed at obtaining new biosystems, establishing and demonstrating the properties of final products and facilitating technology transfer. This goal will be achieved by manufacturing a final product consisting of a biocompatible drug delivery system and evaluating “in vitro” biological interactions along with the biochemical stability and drug release kinetics, bringing the concept closer to industrial application.
GO 3. Consolidation and protection of intellectual property rights in relation to developed materials.
- Realization of a literature study to identify the most recent aspects published in the MMMs domain;
- Preliminary studies for synthesis of MMMs on the basis of silicon as a storage for different molecules, with more capacitance of loading and a regulatory eliberation mechanism;
- Preliminary studies on characterization, in terms of morphological and structural properties of MMMs (FTIR, SAXS, XRD, BET, SEM, ICP-MS, TEM, thermal analysis);
- Preliminary studies will allow the biological evaluation of natural or synthetic MMMs.
- Synthesis of silicon-based MMMs as storage sources of various molecules, with high loading capacity and adaptive release mechanisms;·
- Induction of porosity and size depending on the synthesis conditions (template agents, pH, phase diagram, etc.) to selectively load hydrophobic or hydrophilic substances;·
- Morphological and structural characterization of the obtained MMMs (FTIR, SAXS, XRD, BET, SEM, ICP-MS, TEM, thermal analysis);· Biological evaluation of natural or synthetic MMMs;·
- Development of protocols for characterizing silanized systems under controlled conditions (FTIR, DTA-TG, BET);·
- Loading and release of biologically active agents;·
- Assessing the loading and delivery capacity of medicines;·
- Biological evaluation performed to establish the cytotoxic and antimicrobial activity of the biologically active agents used and their influence on the intestinal microbiota.
- Optimization of protocols according to existing data;·
- Co-charging of bivalent ions and biologically active agents for the development of MMMs charged with biologically active agents with improved properties;·
- Development of porous systems with “closed pore” morphology, loaded with biologically active agents;·
- Synthesis of magnetite in the pores of MMMs, followed by loading with biologically active agents;·
- Evaluation of triggered MMMs (FTIR, SEM, BET, XRD, DTA-TG);· Evaluation of the release profile;·
- Completion of studies on the biological evaluation performed to establish the cytotoxic and antimicrobial activity of the biologically active agents used and their influence on the intestinal microbiota;
Partner (P1) – National Institute for Research & Development in Chemistry and Petrochemistry – ICECHIM
Partner (P2) – Stefan S. Nicolau Institute of Virology (IVN)